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Role of oral streptococci in the pH-dependent carious dentin
https://tmdu.repo.nii.ac.jp/records/195
https://tmdu.repo.nii.ac.jp/records/195bcb0fa6e-ef9d-4e29-b993-7e153ffb4e23
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
04_maeda (780.9 kB)
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Copyright © 2006 Tokyo Medical and Dental University (TMDU)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-08-14 | |||||
| タイトル | ||||||
| タイトル | Role of oral streptococci in the pH-dependent carious dentin | |||||
| タイトル | ||||||
| タイトル | Role of oral streptococci in the pH-dependent carious dentin | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pH | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | carious dentin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | S. mutans | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | oral streptococci | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | pH | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | carious dentin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | S. mutans | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | oral streptococci | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
前田, 朋子
× 前田, 朋子× 北迫, 勇一× 泉福, 英信× Burrow, Michael F.× 田上, 順次 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of the present study was to investigate the extent to which oral streptococci or lactic acid producing bacteria are able to regulate pH value, especially at low pH values associated with dentinal carious activity using a PCR method. One millimeter-thick sections were sliced from 24 extracted carious human molars. The pH values on the tooth sections were evaluated using a pH-imaging microscope. A dentin sample (1×1×1mm) was prepared from the areas with the lowest or highest pH and homogenized to extract bacterial genomic DNA. Specific primers were used for nested PCR to mutans streptococci (MS: Streptococcus mutans and Streptococcus sobrinus). Conserved primers were also used for PCR to lactobacilli and gram positive bacteria. The PCR products were separated by electrophoresis, and then oral bacteria were identified. There was no significant difference between carious and intact dentin in MS identification. However, the frequency of the product amplified by the conserved primers in carious dentin (16/24, 66.7%) was significantly higher than that in intact dentin (2/24, 8.3%), and PCR products demonstrated, by sequence analysis, various bacteria, including oral streptococci. It was concluded that oral streptococci may be associated with the development of "low pH-carious dentin". | |||||
| 書誌情報 |
Journal of Medical and Dental Sciences en : Journal of medical and dental sciences 巻 53, 号 3, p. 159-166, 発行日 2006-09 |
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| 出版者 | ||||||
| 出版者 | 国立大学法人 東京医科歯科大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2185-9132 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12028964 | |||||
| 論文ID（NAID） | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | NAID | |||||
| 関連識別子 | 110006318485 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.11480/jmds.530304 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
