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Glucocorticoids induced the production and gene expression of IL-1α through AP-1 and partially NF-κB activation in murine epidermal cells
https://tmdu.repo.nii.ac.jp/records/292
https://tmdu.repo.nii.ac.jp/records/29260084fe9-18cd-4ea4-94e8-d1b680b081c2
名前 / ファイル | ライセンス | アクション |
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04_Awad (1.3 MB)
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Copyright © 2002 Tokyo Medical and Dental University (TMDU)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-08-16 | |||||
タイトル | ||||||
タイトル | ||||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Keratinocytes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | contact hypersensitivity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytokines | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glucocorticoids | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | signal transduction | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Keratinocytes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | contact hypersensitivity | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cytokines | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | glucocorticoids | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | signal transduction | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Awad , Sherif
× Awad , Sherif× 横関, 博雄× 宮崎, 安洋× 井川, 健× 湊原, 一哉× 佐藤, 貴浩× 西岡, 清 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To investigate the mechanism of the glucocorticoids- induced augmentation of skin response, we have recently reported the modulatory effect of glucocorticoids on the regulation of cytokines production in keratinocytes stimulated with various chemicals in vitro through both NF-κB and AP-1 activation. Further to clarify the mechanism in the glucocorticoids-induced augmentation of cytokines production from keratinocytes, we examined the effect of glucocorticoids to keratinocytes without chemical stimulation. Glucocorticoids 10–4 M inhibited the production of IL-1α from Pam 212 cells. However, lower concentration (10–8–10–10 M) of glucocorticoids significantly enhanced the production of IL-1α by Pam 212 cells at both the protein and mRNA levels. In contrast, glucocorticoids had no effect on the production of either TNF-α, IL-6, nor GM-CSF by Pam 212 cells cultured for 6 h. Electrophoretic mobility shift assays (EMSA) revealed that 10–10–10–12 M glucocorticoids induced the NF-κB activation in Pam 212 cells, however, augmented AP-1 activation by 10–8–10–10 M of glucocorticoids was observed in Pam 212 cells. Furthermore, pyrrolidine dithiocarbamate (PDTC) partially inhibited the IL-1α production and completely inhibited NF-κB expression by Pam 212 cells. On the other hand, MAP-kinase inhibitors (PD98059, SB202190) completely abrogated not only AP-1 activation but the low concentration glucocorticoids-induced IL-1α production. These data indicated that lower concentration of glucocorticoids induced the augmentation of IL-1α production from keratinocytes mediated through the AP-1 pathway and partially through NF-κB pathway. | |||||
書誌情報 |
Journal of Medical and Dental Sciences en : Journal of Medical and Dental Sciences 巻 49, 号 1, p. 27-35, 発行日 2002-03 |
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出版者 | ||||||
出版者 | 国立大学法人 東京医科歯科大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2185-9132 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 130005434753 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 12160224 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.11480/jmds.490104 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |