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Optimal initial cell density that yields the highest number of primary synovial mesenchymal stem cells in a clinical setting
https://tmdu.repo.nii.ac.jp/records/787
https://tmdu.repo.nii.ac.jp/records/78724106a4c-bb3d-4614-95c0-e386cfcd2769
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
03_Horiuchi (3.3 MB)
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Copyright ©2021 Tokyo Medical and Dental University (TMDU)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2021-01-29 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | mesenchymal stem cell | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | synovium | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | initial cell density | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | primary cell | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | human serum | |||||||||||||||||||||
| 公開日 | ||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 著者 |
Horiuchi, Kiyotaka
× Horiuchi, Kiyotaka
× Mizuno, Mitsuru
× Katano, Hisako
× Endo, Kentaro
× Ozeki, Nobutake
× Tsuji, Kunikazu
× Koga, Hideyuki
× Sekiya, Ichiro
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Synovial mesenchymal stem cell (MSC) transplantation improved clinical outcomes in knees. In clinical situations, nucleated cells were cultured with 10% autologous serum for 14 days. Nucleated cell number and serum volume practically varied by patient, and initial cell density and dish number affected yield/donor. Our purpose was to estimate the MSC yield/donor from the fold increase obtained in this experiment in several patterns. We obtained the data of the autologous serum volume, nucleated cell number, and initial plating cell density from 10 patients who participated in our previous clinical studies. We used these previous clinical data and the current experimental data. Experimentally, fold increase significantly decreased between 700 and 1,900 cells/cm². In cases where the nucleated cell number was low and the serum volume was high, the estimated MSC yield/donor decreased along with the initial plating density. In cases where the nucleated cell number was high and the serum volume was low, the estimated MSC yield/donor increased along with the initial plating density. In cases where some nucleated cells were discarded, the maximum MSC yield/donor were obtained when the initial cell density was the highest cell density where some nucleated cells were discarded. | |||||||||||||||||||||
| 書誌情報 |
Journal of Medical and Dental Sciences 巻 68, p. 17-26, 発行日 2021-01 |
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| 出版者 | ||||||||||||||||||||||
| 出版者 | Tokyo Medical and Dental University | |||||||||||||||||||||
| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 2185-9132 | |||||||||||||||||||||
| DOI | ||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | 10.11480/jmds.680003 | |||||||||||||||||||||
