@article{oai:tmdu.repo.nii.ac.jp:00000292,
 author = {Awad , Sherif and Awad , Sherif and 横関, 博雄 and Yokozeki, Hiroo and 宮崎, 安洋 and Miyazaki, Yasuhiro and 井川, 健 and Igawa, Ken and 湊原, 一哉 and Minatohara, Kazuya and 佐藤, 貴浩 and Satoh, Takahiro and 西岡, 清 and Nishioka, Kiyoshi},
 issue = {1},
 journal = {Journal of Medical and Dental Sciences, Journal of Medical and Dental Sciences},
 month = {Mar},
 note = {To investigate the mechanism of the glucocorticoids- induced augmentation of skin response, we have recently reported the modulatory effect of glucocorticoids on the regulation of cytokines production in keratinocytes stimulated with various chemicals in vitro through both NF-κB and AP-1 activation. Further to clarify the mechanism in the glucocorticoids-induced augmentation of cytokines production from keratinocytes, we examined the effect of glucocorticoids to keratinocytes without chemical stimulation. Glucocorticoids 10–4 M inhibited the production of IL-1α from Pam 212 cells. However, lower concentration (10–8–10–10 M) of glucocorticoids significantly enhanced the production of IL-1α by Pam 212 cells at both the protein and mRNA levels. In contrast, glucocorticoids had no effect on the production of either TNF-α, IL-6, nor GM-CSF by Pam 212 cells cultured for 6 h. Electrophoretic mobility shift assays (EMSA) revealed that 10–10–10–12 M glucocorticoids induced the NF-κB activation in Pam 212 cells, however, augmented AP-1 activation by 10–8–10–10 M of glucocorticoids was observed in Pam 212 cells. Furthermore, pyrrolidine dithiocarbamate (PDTC) partially inhibited the IL-1α production and completely inhibited NF-κB expression by Pam 212 cells. On the other hand, MAP-kinase inhibitors (PD98059, SB202190) completely abrogated not only AP-1 activation but the low concentration glucocorticoids-induced IL-1α production. These data indicated that lower concentration of glucocorticoids induced the augmentation of IL-1α production from keratinocytes mediated through the AP-1 pathway and partially through NF-κB pathway.},
 pages = {27--35},
 volume = {49},
 year = {2002}
}